tgf-β monoclonal mouse neutralizing antibody tgfmab clone 1d11  (Bio X Cell)

Journal: Experimental & molecular medicine

Article Title: Insulin resistance mediates high-fat diet-induced pulmonary fibrosis and airway hyperresponsiveness through the TGF-β1 pathway.

doi: 10.1038/s12276-019-0258-7

Figure Lengend Snippet: Fig. 3 AHR and inflammatory features of naive HFD and OVA-HFD models. AHR to MCh (a), inflammatory cell profile in the BALF (b), IL-4 (c) and IL-5 levels (d), and TGF-β1 (e) concentrations in lung homogenates. Immunohistochemical staining for TGF-β1 (F) in tissue from naïve HFD, OVA-HFD, naive ND, and OVA-ND mice are shown. †P < 0.05; ‡P < 0.01; *P < 0.001. AHR airway hyperresponsiveness, MCh methacholine, BALF bronchoalveolar lavage fluid, HFD high-fat-diet, IL interleukin, OVA ovalbumin, ND normal diet, TGF transforming growth factor

Article Snippet: To block TGF-β1, a rabbit anti-mouse TGF-β neutralizing mAb (100 μg/mouse; R&D Systems Inc., Minneapolis, MN, USA) or rabbit IgG antibody (100 μg/ mouse; R&D Systems) was administered once via the intravenous (i.v.) route 4 h before the first OVA challenge (Fig. 1).

Techniques: Immunohistochemical staining, Staining

Journal: Molecular Vision

Article Title: Inhibitory effect of blocking TGF-β/Smad signal on injury-induced fibrosis of corneal endothelium

doi:

Figure Lengend Snippet: Effects of exogenous EGF, TGF-β1, or TGF-β2 on the spreading of the corneal endothelium in organ culture. Exogenous EGF promoted endothelium spreading. While adding exogenous TGF-β1 (A) or TGF-β2 (B) to the medium exhibited no effect on endothelium sheet elongation, it counteracted the promotion of endothelial sheet elongation by exogenous EGF.

Article Snippet: To examine the role of endogenous TGF-β in endothelium spreading, the medium was supplemented with either mouse monoclonal anti-human TGF-β neutralizing antibody (20 μg/ml, R&D systems) or mouse IgG1 derived from BALB/c strain (20 μg/ml, Sigma-Aldrich).

Techniques: Organ Culture

Journal: Molecular Vision

Article Title: Inhibitory effect of blocking TGF-β/Smad signal on injury-induced fibrosis of corneal endothelium

doi:

Figure Lengend Snippet: The role of endogenous TGF-β was evaluated by using neutralizing antibody. Adding neutralizing anti-TGF-β antibody retarded endothelium repair.

Article Snippet: To examine the role of endogenous TGF-β in endothelium spreading, the medium was supplemented with either mouse monoclonal anti-human TGF-β neutralizing antibody (20 μg/ml, R&D systems) or mouse IgG1 derived from BALB/c strain (20 μg/ml, Sigma-Aldrich).

Techniques:

Journal: Molecular Vision

Article Title: Inhibitory effect of blocking TGF-β/Smad signal on injury-induced fibrosis of corneal endothelium

doi:

Figure Lengend Snippet: Roles of TGF-β related signaling on the spreading of the corneal endothelium in organ culture. The p38 inhibitor, SB203580, and the MAPK inhibitor, U0126, hindered endothelium sheet elongation (inhibition: SB203580>U0126). Neither the JNK inhibitor nor the ALK5 inhibitor, SB431542, (blocking Smad2/3 signal) have a significant effect on its repair.

Article Snippet: To examine the role of endogenous TGF-β in endothelium spreading, the medium was supplemented with either mouse monoclonal anti-human TGF-β neutralizing antibody (20 μg/ml, R&D systems) or mouse IgG1 derived from BALB/c strain (20 μg/ml, Sigma-Aldrich).

Techniques: Organ Culture, Inhibition, Blocking Assay